Cell culture medium, specifically used for enhancing sperm motility, such as spermatozoa from frozen-thawed, testicular sperm extraction (TESE) specimens, thus maximizing recovery of motile spermatozoa, potentially increasing fertilization rate in intra-uterus insemination (IUI) and in vitro fertilization (IVF), consequently increasing pregnancy in human and mammal in vitro fertilization (IVF)